Chemistry · Chromatography
Consider the following statements about High-Performance Liquid Chromatography (HPLC): A. In normal phase HPLC, the stationary phase is polar and the mobile phase is non-polar. B. In normal phase HPLC, the least polar compound elutes first and increasing the polarity of the mobile phase decreases the elution time. C. In reverse phase HPLC, the most polar compound elutes first and increasing the polarity of the mobile phase increases the elution time. D. In the isocratic mode of HPLC analysis, the composition and polarity of the mobile phase remain unchanged throughout the run. E. The resolution of analysis depends on a proper balance of intermolecular forces among the analyte, the mobile phase, and the stationary phase. Choose the correct answer from the options given below:
- 1B, C, D and E Only✓
- 2A, B, C and D Only
- 3A, C, D and E Only
- 4B, C and D Only
Correct answer
B, C, D and E Only
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