Biology · Biochemistry
Chromatin immunoprecipitation (ChIP) and RT-PCR were performed to investigate transcriptional activation of a specific gene. Histone acetylation marks H4K8Ac and H3K9Ac as well as TATA-binding protein (TBP) recruitment occur in a defined chronological order. Which combination correctly identifies X, Y, and Z proteins in sequence of gene activation?
- 1X: H3K9Ac; Y: TBP; Z: H4K8Ac
- 2X: H4K8Ac; Y: H3K9Ac; Z: TBP
- 3X: TBP; Y: H4K8Ac; Z: H3K9Ac
- 4X: H4K8Ac; Y: TBP; Z: H3K9Ac✓
Correct answer
X: H4K8Ac; Y: TBP; Z: H3K9Ac
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