Biology · Molecular Biology
Bacterial ribosomes were fractionated using sucrose density gradient centrifugation to separate 30S, 50S, and 70S populations. Incubation with EF-G, protein X, or GTP individually caused no changes. Incubations with EF-G + GTP or protein X + GTP also left the profile intact. However, incubation with protein X, EF-G, and GTP together led to a significant decrease in the 70S peak along with a corresponding increase in the 30S and 50S peaks. What is the correct conclusion?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1EF-G binds GTP, proving that the entire effect of GTP is mediated solely through EF-G while protein X does not interact with the nucleotide.
- 2Protein X independently binds and hydrolyzes GTP to promote subunit splitting.
- 3Protein X is a ribosome dissociation factor that functions in cooperation with EF-G and GTP.✓
- 4Protein X is an anti-association factor that functions exclusively in the presence of EF-G and GTP.
Correct answer
Protein X is a ribosome dissociation factor that functions in cooperation with EF-G and GTP.
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