Biology · Biotechnology: Principles and Processes
Assertion (A): Taq polymerase is not the only thermostable DNA polymerase used in PCR; other enzymes like Pfu polymerase (from Pyrococcus furiosus) and Vent/Tli polymerase (from Thermococcus litoralis) are also employed. Reason (R): These polymerases are thermostable and possess high fidelity (proofreading activity), making them suitable for repeated heating cycles in PCR. Choose the correct option:
- 1Assertion (A) is false, but Reason (R) is true
- 2Assertion (A) is true, but Reason (R) is false
- 3Both Assertion (A) and Reason (R) are true, but Reason (R) is not the correct explanation of Assertion (A)
- 4Both Assertion (A) and Reason (R) are true, and Reason (R) is the correct explanation of Assertion (A)✓
Correct answer
Both Assertion (A) and Reason (R) are true, and Reason (R) is the correct explanation of Assertion (A)
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