Biology · Biotechnology
Arrange the following steps of a standard recombinant DNA-cloning procedure in the correct chronological sequence of their occurrence: A. Joining the target DNA fragment with a cleaved cloning vector using DNA ligase. B. Cleaving both the target DNA from the source organism and the cloning vector with the same restriction endonuclease. C. Selection of transformants from non-transformants using antibiotic resistance markers or color selection indicators. D. Introduction of the recombinant DNA molecule into a competent host cell. Choose the correct sequence from the options given below:
- 1C, B, D, A
- 2B, A, D, C✓
- 3A, C, B, D
- 4A, B, C, D
Correct answer
B, A, D, C
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