Biology · Biotechnology

An experiment was performed to introduce a transgenic trait in a crop plant by Agrobacterium-mediated transformation using a transgene construct in which the transgene was expressed using the CaMV 35S promoter. It was observed that expression levels of the transgenic protein were very low in all transgenic plants while transgene mRNA levels were high and variable among different plants. Further, different transgenic lines contained different numbers of the T-DNA insert. The following statements were made to explain the above observation: A. Variations in the number of T-DNA inserts in different transgenic plants is due to more number of host cells getting infected with the T-DNA. B. Low expression levels of the transgenic protein in all transgenic plants could be due to codon usage variations between the host plant and the heterologous source of the transgene. C. The coding sequence of the transgene contained sequences that destabilized the transgene mRNA. D. Variation in copy number of T-DNA in different transgenic plants is due to variation in the promoter used to express the transgene. Which one of the following options represents all correct statements?