Biology · Enzyme Kinetics

An enzyme-catalyzed reaction comparing proton and deuteron transfer reactions yielded the following kinetic data: k cat [H] = 70.7 s -1 K M [H] = 1.03 mM k cat [D] = 10.3 s -1 K M [D] = 1.06 mM Which of the following represents the specificity constants (M -1 s -1 ) for proton transfer, deuteron transfer, and the kinetic isotope effect, respectively?