Biology · Biotechnology
A student successfully cloned a DNA fragment flanked by BamHI (5'-G↓GATCC-3') sites into a vector cleaved with BglII (5'-A↓GATCT-3') due to compatible cohesive ends. What are the correct restriction cleavage sites for these two enzymes (where '/' indicates the point of enzymatic cleavage)?
- 1GGATC/C and AGA/TCT
- 2GGATC/C and A/GATCT
- 3G/GATCC and A/GATCT✓
- 4G/GATCC and AGATC/T
Correct answer
G/GATCC and A/GATCT
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