Biology · Biotechnology
A scientist wants to produce human insulin using E. coli. She isolates the insulin gene, amplifies it using PCR, and inserts it into a plasmid vector carrying an ampicillin resistance gene. The plasmid is then introduced into E. coli, and the transformed cells are cultured in a medium containing ampicillin. Why will only the transformed E. coli survive in the medium?
- 1Only transformed cells produce insulin.
- 2Only transformed cells carry the ampicillin resistance gene.✓
- 3Only transformed cells can replicate plasmids.
- 4Only transformed cells can utilize glucose in the medium.
Correct answer
Only transformed cells carry the ampicillin resistance gene.
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