Biology · Biotechnology Principles and Processes
A scientist inserts a foreign DNA fragment into the tetracycline resistance gene of plasmid pBR322 at a BamHI site. The transformed bacteria are first grown on ampicillin medium and then transferred to tetracycline medium. Which observation confirms recombinant bacteria?
- 1No growth on both media
- 2Growth on ampicillin but no growth on tetracycline✓
- 3No growth on ampicillin but growth on tetracycline
- 4Growth on both ampicillin and tetracycline
Correct answer
Growth on ampicillin but no growth on tetracycline
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