Biology · Recombinant DNA Technology
A researcher isolates a restriction enzyme that recognizes the sequence 5'-GAATTC-3'. After digestion, the DNA fragments show single-stranded overhangs. These fragments are later joined using DNA ligase. Which of the following best explains the role of the enzyme used?
- 1It cuts DNA randomly producing blunt ends
- 2It recognizes palindromic sequences and produces sticky ends✓
- 3It removes nucleotides from DNA ends
- 4It methylates DNA to prevent cutting
Correct answer
It recognizes palindromic sequences and produces sticky ends
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