Biology · Biochemistry and Thermodynamics
A protein with a binding pocket containing amino acid residues Asp (pKa = 4.0) and His (pKa = 6.5) interacts with a ligand at pH 7.0 at 25 °C. The binding affinity of the ligand increases 10-fold upon 'His' protonation. Assuming 'His' protonation is the sole contributor to the enhanced binding, calculate the fraction of 'His' residue protonation and the change in the binding free energy corresponding to the enhanced affinity (R = 1.987 cal/mol/K).
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 10.24 and -1.36 kcal/mol✓
- 20.96 and -1.63 kcal/mol
- 30.76 and -2.46 kcal/mol
- 40.48 and -3.61 kcal/mol
Correct answer
0.24 and -1.36 kcal/mol
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