Biology · Immunological Techniques
A mixed cell population was stained with two antibodies: an anti-A antibody specific for cell surface antigen A (labeled with fluorescein, emitting green fluorescence) and an anti-B antibody specific for cell surface antigen B (labeled with rhodamine, emitting red fluorescence). When analyzing this stained population using a standard two-color flow cytometry dot plot, how are the different cell sub-populations represented?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1All cells will cluster into a single quadrant in the upper left corner regardless of expression
- 2Cells expressing only antigen A appear in one quadrant, cells expressing only antigen B appear in another, double-positive cells appear in a third quadrant, and double-negative cells appear in the fourth quadrant✓
- 3Fluorescence intensity cannot separate the populations, so all cells form a continuous diagonal single line
- 4Flow cytometry can only detect rhodamine signal, so green fluorescein-positive cells remain completely undetected
Correct answer
Cells expressing only antigen A appear in one quadrant, cells expressing only antigen B appear in another, double-positive cells appear in a third quadrant, and double-negative cells appear in the fourth quadrant
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