A group of researchers tested two selection agents, M1 and M2, for selecting transgenic plants using three explants (A, B, and C) without Agrobacterium transformation. The regeneration frequencies (%) obtained were: - Explant A: M1 (20, 60, 100 mg/L) gave 44%, 21%, Nil; M2 (10, 15, 20 mg/L) gave 8%, Nil, Nil. - Explant B: M1 gave 53%, 30%, 10%; M2 gave Nil, Nil, Nil. - Explant C: M1 gave 71%, 42%, 18%; M2 gave 15%, 9%, 4%. Based on this data, which of the following conclusions is INCORRECT?

Question

Biology · Plant Biotechnology

Verified Solution4 Options

Answer 1

M2 is a stronger selection agent than M1 for explant types A and B.

Answer 2

A concentration of 100 mg/L of M1 and 15 mg/L of M2 can be used for selection of transformed cells using explant type A.

Answer 3

Among the three explants, type C is the least suitable at the tested concentrations of M1 and M2.

Answer 4

The use of M1 with explant type B is the most ideal combination for the selection of transformants.

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