Biology · Plant Biotechnology
A group of researchers tested two new agents, M1 and M2, for selecting transgenic plants using three explants (A, B, and C) without Agrobacterium transformation. The regeneration frequencies (%) obtained were: Explant type M1 conc. (mg/L) M2 conc. (mg/L) 20 60 100 10 15 20 A 44% 21% Nil 8% Nil Nil B 53% 30% 10% Nil Nil Nil C 71% 42% 18% 15% 9% 4% Based on the data, which of the following conclusions is INCORRECT?
- 1Use of M1 with explant type B is the most ideal combination for selection of transformants.✓
- 2Among the three explants, type C is least suitable at the tested concentrations of M1 and M2.
- 3A concentration of 100 mg/L of M1 and 15 mg/L of M2 can be used for selection of transformed cells using explant type A.
- 4M2 is a stronger selection agent than M1 for explant types A and B.
Correct answer
Use of M1 with explant type B is the most ideal combination for selection of transformants.
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