Biology · Biophysical Chemistry
A 100-residue long protein has a single chromophoric residue (tyrosine). The UV absorption of this protein and a homologous protein (also with a single tyrosine residue) was monitored at 280 nm at different pH conditions. A plot of the absorbance as a function of pH shows a significant difference in their titration curves. Which one of the following rationalizes the difference in the two pH titrations?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1Hydrolysis of the polypeptide as a function of pH
- 2pH-dependent changes in the absorption in the polypeptide main chain
- 3Location of the tyrosine residue in the protein structure✓
- 4Removal of the hydroxyl group of tyrosine above pH 11
Correct answer
Location of the tyrosine residue in the protein structure
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